Parasites, Infection and stool test ‘Oh No’! Not to worry! The GI-MAP can be used in the detection and identification of gastrointestinal microbial nucleic acids and has been clinically validated for the detection of gastrointestinal pathogens that cause infectious colitis or gastroenteritis. This technology has been used to identify and control pathogen outbreaks because of its rapid turn-around-time.
It measures a substantial list of opportunistic pathogens as well as a list of FDA-cleared pathogens, including novel targets such as viruses, Microsporidia, and pathogenic virulence factors. Chronic gastrointestinal symptoms, intestinal permeability, hormonal imbalance, and food sensitivities may trace their origins to imbalanced gut microbes as a root cause.
The more we look, the more we learn! For example, research is showing a strong link between arthritis and the microbial content of the gut in those that suffer from this debilitating disease process.
Consider this, we now know that there is a strong correlation between depression and the gut microbiome. Simply put, the more we look, the more we reaffirm the fact that gut microbiota is essential to human health and wellbeing. The brain talks to the gut and the gut talks to the brain. It is no longer a news flash that the microbiota in the gut participates in the bidirectional communication between the gut and the brain.
Since the immune system, the intestinal barrier, and microbial diversity are intimately interwoven, thorough understanding of our gut microbiome holds promise for new approaches to treat and prevent disease.
What Makes The GI-MAP™ Different?
✔️ Automated, multiplex DNA (PCR) analysis method, allowing for the simultaneous measurement of multiple bacteria, fungi, parasites, and viruses (more info below)
Single stool sample
✔️ Using DNA sequencing allows for superior sensitivity and specificity in the detection of 15 of the most common causes of gastroenteritis, as well as other chronic diseases
✔️ Intestinal health markers allow for a comprehensive analysis of your gut microbiome, together with markers of inflammation, mucosal immune system and digestion
✔️ Evidence-based and transparent: all data available in the fully-referenced 26-page White Paper (Click Here)
Complete List of Target Analytes Measured on the GI-MAP
C. diff Toxin A & B**
E. coli o157**
Enterotoxigenic E. coli LT & ST (ETEC)**
Shiga-like Toxin producing E. coli stx1 & stx2 (STEC)**
Adenovirus 40 & 41**
Norovirus GI & GII**
Helicobacter pylori and virulence factors, cagA and vacA**
Bacteroides fragilis grp
E. coli (total)
Difference between Multiplex Polymerase Chain Reaction (PCR) and Culture Techniques
Multiplex PCR makes it possible to simultaneously detect many different organisms in one sample. The automated nature of this method minimizes the chance for human error; indeed, this is the only FDA-cleared DNA test for gastrointestinal microbes and pathogens available.
Multiplex polymerase chain reaction (PCR) means that many genes are amplified at the same time, as though many separate PCR reactions were happening at once. This technique makes it possible to simultaneously detect many different organisms in one sample. Multiple primers and probes for each organism allow for enhanced sensitivity and specificity. The method measures the 16S or 23S ribosomal RNA (rRNA) regions, virulence factors, and viral targets for microbial detection.
Other stool tests on the market primarily rely on bacterial culture of the stool specimen. A limitation of this method is that only the organisms that grow can be identified, meaning anaerobic organisms and parasites that do not grow under routine culture conditions cannot be identified. This is particularly prevalent with anaerobes like Lactobacillus. Often results of ‘NG’ or ‘0+’ are reported, but it obviously does not mean it is not present, just simply that it would not grow in the lab, which is also seen with many yeasts and fungi.
Turn Around Time: 10 – 12 days